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Schematic graph showing the expected trends in Cornulin expression as dysplastic growth progresses toward malignant forms of oral squamous cell carcinoma. This original figure was drawn by authors using Microsoft Office PowerPoint and BioRender software.

Journal: Cureus

Article Title: Characterization of Cornulin as a Molecular Biomarker for the Progression of Oral Squamous Cell Carcinoma

doi: 10.7759/cureus.32210

Figure Lengend Snippet: Schematic graph showing the expected trends in Cornulin expression as dysplastic growth progresses toward malignant forms of oral squamous cell carcinoma. This original figure was drawn by authors using Microsoft Office PowerPoint and BioRender software.

Article Snippet: The squamous cell carcinoma 25 (SCC25) human OSCC cell line, Detroit 562 metastatic pharyngeal squamous cell carcinoma (SCC), and primary gingival epithelial cells were acquired from American Type Culture Collection (ATCC) (Manassas, VA), and the dysplastic oral keratinocytes (DOKs) were acquired from the European Collection of Authenticated Cell Cultures (ECACC) (Salisbury, United Kingdom) via Millipore Sigma (Darmstadt, Germany).

Techniques: Expressing, Software

Cell line descriptions, special characteristics, culture media, and sources.

Journal: Cureus

Article Title: Characterization of Cornulin as a Molecular Biomarker for the Progression of Oral Squamous Cell Carcinoma

doi: 10.7759/cureus.32210

Figure Lengend Snippet: Cell line descriptions, special characteristics, culture media, and sources.

Article Snippet: The squamous cell carcinoma 25 (SCC25) human OSCC cell line, Detroit 562 metastatic pharyngeal squamous cell carcinoma (SCC), and primary gingival epithelial cells were acquired from American Type Culture Collection (ATCC) (Manassas, VA), and the dysplastic oral keratinocytes (DOKs) were acquired from the European Collection of Authenticated Cell Cultures (ECACC) (Salisbury, United Kingdom) via Millipore Sigma (Darmstadt, Germany).

Techniques: Mutagenesis

(A) Western blot showing expression of Cornulin and the GAPDH loading control in the four cell lines tested. (B) Comparison of Cornulin expression relative to GAPDH expression in normal oral keratinocytes (PGK), dysplastic oral keratinocytes (DOK), locally invasive oral squamous cell carcinoma cells (SCC25), and metastatic oral squamous cell carcinoma cells (Detroit 562). Error bars represent standard error of the mean (SEM), and values shown above brackets indicate significant p-values pertaining to the difference of expression between cell lines. GAPDH, glyceraldehyde 3-phosphate dehydrogenase

Journal: Cureus

Article Title: Characterization of Cornulin as a Molecular Biomarker for the Progression of Oral Squamous Cell Carcinoma

doi: 10.7759/cureus.32210

Figure Lengend Snippet: (A) Western blot showing expression of Cornulin and the GAPDH loading control in the four cell lines tested. (B) Comparison of Cornulin expression relative to GAPDH expression in normal oral keratinocytes (PGK), dysplastic oral keratinocytes (DOK), locally invasive oral squamous cell carcinoma cells (SCC25), and metastatic oral squamous cell carcinoma cells (Detroit 562). Error bars represent standard error of the mean (SEM), and values shown above brackets indicate significant p-values pertaining to the difference of expression between cell lines. GAPDH, glyceraldehyde 3-phosphate dehydrogenase

Article Snippet: The squamous cell carcinoma 25 (SCC25) human OSCC cell line, Detroit 562 metastatic pharyngeal squamous cell carcinoma (SCC), and primary gingival epithelial cells were acquired from American Type Culture Collection (ATCC) (Manassas, VA), and the dysplastic oral keratinocytes (DOKs) were acquired from the European Collection of Authenticated Cell Cultures (ECACC) (Salisbury, United Kingdom) via Millipore Sigma (Darmstadt, Germany).

Techniques: Western Blot, Expressing, Control, Comparison